Period of performance:
Marine aquaculture is thought to be vital for ensuring food supply for the future but like no other food production system, it is subject to catastrophic collapse due to rapidly spreading pathogens. The bacterial genus Vibrio is most commonly involved in aquaculture infections and is likely to become even more prevalent due to their positive response to warming ocean surface water. There are currently no viable treatments for vibrios in aquaculture systems since antibiotic application in aquatic systems is ineffective and causes problems due to selection for resistance. Here we propose to develop and test bacterial viruses, bacteria’s natural enemies, as an effective and rapid treatment for pathogen infections in aquaculture systems. Our approach is based on our experience with vibrios and their viruses and builds on an extensive collection of well-characterized isolates of both bacterial hosts and their specific viruses.
Key to finding a solution is to develop virus cocktails that target populations of genotypically diverse pathogens by combining viruses with varying host range and receptor specificity. The latter is important since it makes buildup of resistance highly improbable in the time course of treatment. Viral receptors are frequently surface proteins that play a vital role in the biology of bacteria and resistance is often associated with their mutational loss or decreased function. Hence bacteria with multiple virus resistance mutations are less competitive in the environment and should be diluted from microbial communities.
Our specific aims are therefore to use our existing large collection of Vibrio hosts and viruses to characterize receptors that allow different virus families to infect, verify the host range of these different virus families, and assemble and test virus cocktails for their effectiveness in killing pathogen populations in laboratory and field experiments. This proof of concept work will be carried out in oysters and Vibrio pathogens that infect them through collaboration with colleagues in France where up to 90% of juvenile oysters are currently killed by V. crassostreae. The final product of this research will be a tested virus cocktail that can be relatively easily be produced in large quantities and applied in the field. Because we work with natural isolates, no regulatory issues of using biologically engineered organisms are expected. We also envision that the protocols and logic of our approach will be broadly applicable to other aquaculture and agricultural systems affected by recurrent infections of the same pathogen population.